Detailed view for Tb927.6.1850

Basic information

TDR Targets ID: 15659
Trypanosoma brucei, quiescin sulfhydryl oxidase
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.9173 | Length (AA): 526 | MW (Da): 58755 | Paralog Number: 0

Signal peptide: Y | GPI Anchor: | Predicted trans-membrane segments: 1

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00085   Thioredoxin
PF04777   Erv1 / Alr family

Gene Ontology

Mouse over links to read term descriptions.
GO:0016972   thiol oxidase activity  
GO:0055114   oxidation reduction  
GO:0045454   cell redox homeostasis  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There is 1 model calculated for this protein. More info on this model, including the model itself is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
29 445 3qcp (A) 29 446 99.00 0 1 2.01318 -1.47

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

PDB tag
  • 3QCP:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 3QD9:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Procyclic. Siegel TN
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_129038)

Species Accession Gene Product
Arabidopsis thaliana AT1G15020   quiescin-sulfhydryl oxidase 1
Arabidopsis thaliana AT2G01270   quiescin-sulfhydryl oxidase 2
Brugia malayi Bm1_36435   Erv1 / Alr family protein
Caenorhabditis elegans CELE_F47B7.2   Protein F47B7.2, isoform B
Caenorhabditis elegans CELE_T10H10.2   Protein T10H10.2
Caenorhabditis elegans CELE_F35G2.1   Protein F35G2.1, isoform B
Drosophila melanogaster Dmel_CG17843   CG17843 gene product from transcript CG17843-RA
Drosophila melanogaster Dmel_CG6690   CG6690 gene product from transcript CG6690-RA
Drosophila melanogaster Dmel_CG4670   CG4670 gene product from transcript CG4670-RB
Drosophila melanogaster Dmel_CG31413   CG31413 gene product from transcript CG31413-RA
Echinococcus granulosus EgrG_000245600   sulfhydryl oxidase 1
Echinococcus multilocularis EmuJ_000245600   sulfhydryl oxidase 1
Homo sapiens ENSG00000165661   quiescin Q6 sulfhydryl oxidase 2
Homo sapiens ENSG00000116260   quiescin Q6 sulfhydryl oxidase 1
Leishmania braziliensis LbrM.30.0510   quiescin sulfhydryl oxidase, putative
Leishmania donovani LdBPK_300440.1   quiescin sulfhydryl oxidase, putative
Leishmania infantum LinJ.30.0440   quiescin sulfhydryl oxidase, putative
Leishmania mexicana LmxM.29.0430   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_00988   hypothetical protein
Loa Loa (eye worm) LOAG_11834   Erv1/Alr family protein
Loa Loa (eye worm) LOAG_11291   hypothetical protein
Mus musculus ENSMUSG00000033684   quiescin Q6 sulfhydryl oxidase 1
Mus musculus ENSMUSG00000036327   quiescin Q6 sulfhydryl oxidase 2
Oryza sativa 4339541   Os05g0552500
Onchocerca volvulus OVOC2134   Sulfhydryl oxidase homolog
Onchocerca volvulus OVOC4629   Sulfhydryl oxidase homolog
Onchocerca volvulus OVOC3401   Sulfhydryl oxidase homolog
Schistosoma japonicum Sjp_0038080   ko:K10758 thiol oxidase [EC1.8.3.2], putative
Schistosoma mansoni Smp_098710   quiescin q6-related sulfhydryl oxidase
Schmidtea mediterranea mk4.027261.00  
Schmidtea mediterranea mk4.018305.00  
Trypanosoma brucei gambiense Tbg972.6.1550   hypothetical protein, conserved
Trypanosoma brucei Tb927.6.1850   quiescin sulfhydryl oxidase
Trypanosoma congolense TcIL3000_0_58190   quiescin sulfhydryl oxidase
Trypanosoma congolense TcIL3000_6_1440   quiescin sulfhydryl oxidase
Trypanosoma cruzi TcCLB.511491.170   quiescin sulfhydryl oxidase, putative
Trypanosoma cruzi TcCLB.511493.10   quiescin sulfhydryl oxidase, putative

Essentiality

Tb927.6.1850 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.6.1850 this record Trypanosoma brucei significant gain of fitness in bloodstream forms (3 days) alsford
Tb927.6.1850 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.6.1850 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.6.1850 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_F47B7.2 Caenorhabditis elegans slow growth wormbase
Show/Hide essentiality data references
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) increased (PATO:0000470) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in bloodstream forms (stage 3 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

  • Reagent:
  • Target Type Source Notes
    Tb927.6.1850 cloned gene BRENDA A gene with this EC number or name or sequence has been cloned from Trypanosoma brucei ( 1 )

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.6.1850 (Trypanosoma brucei), quiescin sulfhydryl oxidase
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